Effect of free
galactan on recall proliferation of CD4+ T lymphocytes from naïve cattle (
) and CBPP-infected cattle (
). CFSE-labeled cells were stimulated for 7 days with whole inactivated Mmm (T1/44, 5 μg/mL), galactan (EPS, 10 μg/mL) or PPDa (10 μg/mL). Different symbols represent the net effect of stimulations (i.e., stimulated cultures minus non-stimulated cultures) for each animal individually (n = 3). Asterisks indicate statistically significant differences with PPDa.