Effect of free
galactan on surface expression of CD40 (
) and CD80 (
) on bovine macrophages. Cells were incubated for 48 h with galactan (EPS) at 0.1, 1 and 10 µg/mL, or with LPS and medium only (C−) as positive and negative controls respectively. Cell-surface expression of CD40/CD80 was measured by flow cytometry and results expressed as mean (±SD) percentages of positive cells for a given marker (n = 3). Asterisks indicate statistically significant differences (p < 0.05) with the negative control.