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Figure 2 | Veterinary Research

Figure 2

From: Identification and characterisation of microsatellite DNA markers in order to recognise the WSSV susceptible populations of marine giant black tiger shrimp, Penaeus monodon

Figure 2

Microsatellite DNA marker analysis of shrimp and WSSV genomic DNA. Genomic DNA was isolated from pleopod tissue of Penaeus monodon and subjected to microsatellite DNA marker analysis. PCR amplified DNA fragments were electrophoresed in 2.5% agarose gel and photographed after staining with ethidium bromide. In panel A Lanes 1–6 show PCR amplified DNA bands from WSSV resistant P. monodon. Lanes 7–12 show PCR amplified DNA bands from disease susceptible P. monodon. Lane C- is the negative control and Lane M indicates molecular weight marker. Number in the right side indicated the molecular size of molecular weight marker and at the left hand side the molecular weights of the significant bands specific to the susceptible samples are indicated by the arrows in the figure. In panel B WC+ shows the amplified DNA bands in the nested PCR of WSSV genomic DNA with WSSV specific primers, WC- shows no amplified DNA bands in the PCR of WSSV genomic DNA with shrimp specific microsatellite primers (OM 73 and OM 74).

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Veterinary Research

ISSN: 1297-9716