Figure 8

Differential endocytosis of OVA and Dextran by subpopulations of myeloid cells. PBMC were isolated and incubated for 30 min with 100 μg/mL of TexasRed-OVA (A, B) or TexasRed-Dextran (C, D) at 37 °C or on ice (to assess background uptake). The cells were subsequently washed and stained with conjugated CD14 and CD16 antibodies and analysed by flow cytometry. Live, single cells were gated as in Figure 1 and the MFI was calculated for the CD14⁻CD16⁺⁺ and CD14⁺ gated populations (A, C). The results shown (B, D) are mean values with error bars showing ± SE for four animals and represent the TexasRed MFI at 37 °C minus MFI on ice (4 °C).