Figure 5
LPS stimulation of distinct myeloid cell populations induces differential cytokine secretion profiles. Cells were purified by using gating strategy shown in Figure 2C and stimulated for 18 h with LPS (black bars) or medium alone (grey bars). The levels IL-1b (A), TNF-a (B), IL-6 (C), IL-10 (D) and IL-12 (E) secreted into culture supernatants were measured by ELISA. The data is expressed as the concentration of cytokine in picograms (pg) or biological activity (biological units (BU)) secreted by 106 cells. Different letters denote statistical significance between the different cell populations: capital letters represent significant difference of the fold increase induced by stimulation (stimulated value divided by un-stimulated control), while lowercase denotes significance in the comparison of un-stimulated values. Results are shown as the mean values with error bars indicating ± SE of the sorted populations from six animals.