Figure 2

Expression of CD14 and CD16 defines subpopulations of cells in bovine peripheral blood with differential expression. The expression of CD16 and CD14 was determined by flow cytometry in freshly isolated bovine PBMC. Live, single cells gated as in Figure 1, were analysed by staining for CD16 (A) or CD14 (B). To further characterise these populations, live single cells were then assessed for expression of CD16 and CD14 by double staining (C). Variable CD16 expression was observed. As shown in Figure 1 cells with CD16 fluorescence <400 were negative for expression. Cells with a CD16 fluorescence between 800 and 10 000 were NKp46⁺ NK cells (panel Q3). Cells with CD16 fluorescence above 10 000 (panel Q4) were identified as a discrete population of CD172a⁺ NKp46⁻ CD16⁺CD14⁻ cells (CD14⁻CD16⁺⁺). CD14⁺ cells had fluorescence intensity >300 and expressed differential levels of CD16. Two CD14⁺ populations were defined by a diagonal gate (panels Q1 and Q2 [8]). Data shown are for one representative animal (A, B) and for six individual animals (C).