Comparative analysis of rabbit hemorrhagic disease virus (RHDV) and new RHDV2 virus antigenicity, using specific virus-like particles

Table 1 Reactivity of anti-RHDV MAbs against the capsid proteins of RHDV, RHDV2 and EBHSV in indirect ELISA assays.

MAb	RHDV	RHDV2	EBHSV	Epitope location (aa position within VP60 protein)
2E7	+	+	+	(aa 549-579, P1 subdomain)
1G5	+	+	-	NPISQVAP (aa 326-333, loop 2 at P2 subdomain)
1C9^a	+	-	-	PGNNAT (aa 305-310, loop 1 at P2 subdomain)

+ Positive reaction; - Negative reaction.
^a 1C9 is a conformational epitope. It does not bind to RHDV VLPs derived from VP60 deletion or insertion mutants lacking the sequence corresponding to aa positions 305-310 of VP60 protein.

ISSN: 1297-9716