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Figure 6 | Veterinary Research

Figure 6

From: Piscine orthoreovirus (PRV) in red and melanised foci in white muscle of Atlantic salmon (Salmo salar)

Figure 6

Immunohistochemical staining for PRV antigens of red (A-B) and melanised (C-H) focal changes. (A) Abundant amounts of PRV-positive cells (brown) and erythrocytes in the necrotic muscle tissue. (B) Transverse section of necrotic myocytes with intracellular PRV-positive macrophage-like cells and erythrocytes (brown). Distinct granular staining is present in the cytoplasm of the macrophage-like cells. (C) A well-organised granuloma is present in the center of the image, heavily positive for PRV (red), and with vast amounts of elongated melano-macrophages (black). (D) A single granuloma with PRV-positive cells (red) surrounded by heavily pigmented melano-macrophages. (E) A focus with necrotic tissue and infiltrates of melano-macrophages (black) and macrophage-like cells positive for PRV (red). (F) A close-up of E where the distinct reaction in the cytoplasm is evident (red). (G) PRV antigen (red) in melano-macrophages (black) in a necrotic myocyte. Higher resolution image in the upper right corner (100 x). (H) A single necrotic myocyte undergoing phagocytosis and containing abundant PRV-positive macrophage-like cells (red). (A, C) scale bar = 200 μm, (E) scale bar = 100 μm, (B, D, F, G, H) scale bar = 50 μm

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