Figure 2From: Immunization routes in cattle impact the levels and neutralizing capacity of antibodies induced against S. aureus immune evasion proteins IgG1 and IgG2 antibody titer increases in serum and milk. Isotype specific antibody titers in serum (A-D) and milk (E-H) following IN/IN (●), IM/IM (■IMM/SC (▲) or SC/SC (▼) immunization with Efb (A-B, E-F) and LukM (C-D, G-H) were measured by ELISA. Results are expressed as the mean per group ± SEM. Differences in total titer increases following prime plus booster immunizations were analyzed using the One-way ANOVA. Letters indicate significant differences between IN/IN and IM/IM (a), IN/IN and IMM/SC (b), IN/IN and SC/SC (c), IM/IM and SC/SC (d), and IMM/SC and SC/SC (e) immunization routes.Back to article page