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Figure 3 | Veterinary Research

Figure 3

From: Yersinia ruckeri, the causative agent of enteric redmouth disease in fish

Figure 3

Detection methods for Yersinia ruckeri using loop-mediated isothermal amplification method. A: Agarose gel showing ERM-LAMP products of Y. ruckeri; Lane mar: 100 bp DNA ladder, lane Y. ruc: amplified Y. ruckeri LAMP product, lane Y. ruc dig: Hph I digested Y. ruckeri LAMP products of 87 and 108 bp, and lane veco: negative control. B: Visual detection of ERM LAMP products using SYBR Green I stain 1: 1: Negative control reaction using Rox- labelled probe, there is neither pellet nor red fluorescence, 2: positive control reaction using Rox- labelled probe, the pellet emitted red fluorescence; 3: positive sample by using FDR, emitted strong green fluorescence when exposed to UV light; 4: negative sample by using FDR, did not emit strong green fluorescence under UV light; 5: positive sample with green color using SYBR green I stain; 6: negative sample with orange color using SYBR green I stain (Image from Saleh et al. [35] with permission).

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