Detection methods for
using loop-mediated isothermal amplification method. A: Agarose gel showing ERM-LAMP products of Y. ruckeri; Lane mar: 100 bp DNA ladder, lane Y. ruc: amplified Y. ruckeri LAMP product, lane Y. ruc dig: Hph I digested Y. ruckeri LAMP products of 87 and 108 bp, and lane veco: negative control. B: Visual detection of ERM LAMP products using SYBR Green I stain 1: 1: Negative control reaction using Rox- labelled probe, there is neither pellet nor red fluorescence, 2: positive control reaction using Rox- labelled probe, the pellet emitted red fluorescence; 3: positive sample by using FDR, emitted strong green fluorescence when exposed to UV light; 4: negative sample by using FDR, did not emit strong green fluorescence under UV light; 5: positive sample with green color using SYBR green I stain; 6: negative sample with orange color using SYBR green I stain (Image from Saleh et al.  with permission).