Figure 9

Gene transcription of molecules involved in cholesterol de novo synthesis and cholesterol esterification and hydroxylation in E. bovis -infected endothelial host cells. Total RNA was isolated from E. bovis-infected BUVEC (n = 3) at different time points of macromeront formation (12, 14, 17 and 20 days pi), reverse transcribed and subjected to quantitative real-time PCR assays for the detection of bovine acetyl-CoA acyl transferase 1 (ACAT1), acetyl-CoA acyl transferase 2 (ACAT2), HMG-CoA synthase 1 (HMGCS1), HMG-CoA reductase (HMGCR), squalene epoxidase (SQLE), sterol O-acyltransferase 1 (SOAT1), cholesterol 25-hydroxylase (CH25H), low density lipoprotein receptor (LDLR) and oxidized low density lipoprotein receptor 1 (OLR1). Non-infected BUVEC were equally processed at each time point of the experiments and served as negative controls.* = p ≤ 0.05, ** = p ≤ 0.01, *** = p ≤ 0.0001.