Figure 7

LDL-binding and LDL surface receptor abundance on E. bovis -infected endothelial host cells. A-B: E. bovis-infected BUVEC and non-infected controls were cultured in LDL-depleted medium for 36-48 h then incubated in medium containing Bodipy-labelled LDL. After incubation at 4 °C for 1 h followed by 4 h of incubation at 37 °C, LDL binding was measured by FACS analysis: exemplary histogram (A) and LDL quantification in infected (black bar, 17 days pi) and non-infected (white bar) cell layers. C-D: For the quantification of surface LDL receptors, infected (black bar 17 days pi) and non-infected (white bar) cell layers were reacted with LDL receptor (LDLR)-specific primary antibodies and appropriate FITC-conjugated secondary antibodies and subjected to flow cytometry analyses: exemplary histogram (C) and LDLR expression on the surface of E. bovis-infected (black bar, 17 days pi) and non-infected (white bar) BUVEC.