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Figure 2 | Veterinary Research

Figure 2

From: Eimeria bovis infection modulates endothelial host cell cholesterol metabolism for successful replication

Figure 2

Cholesterol distribution and cholesterol content in E. bovis infected host endothelial cells. Bovine umbilical vein endothelial cells (BUVEC) were infected with E. bovis sporozoites and stained with filipin at one day (A; B = phase contrast, arrows indicate sporozoites) and 17days (C; D = phase contrast) pi. Note the bright filipin staining adjacent to intracellular sporozoites (A) and the strong reactions in a meront I-carrying cell (C) compared to non-infected cells in the neighborhood. For the quantification of the total cholesterol contents (E), infected (black bars) and non-infected (grey bars) BUVEC (n = 7) were harvested at different time points after infection (4, 8, 17 days pi), processed for total lipid extraction and total cholesterol measurement using the Amplex Red® kit. Pure sporozoites (processed numbers correspond to the infection dose) were used as controls. Scale bars represent 10 μm.

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