Figure 3
From: Keratin and S100 calcium-binding proteins are major constituents of the bovine teat canal lining
Western blotting of teat canal lining from individual cows. Proteins extracted from the TCL of six individual cows (A-F) were subjected to SDS–PAGE followed by western blot analysis. Samples were collected from a forequarter (F) and hindquarter (H) teat from each cow. The blots were probed with antibodies directed against bovine S100A7/A7-like, S100A8, S100A9, and S100A12. A replicate gel loaded with 10 μg of total protein was stained with Coomassie blue G-250 (bottom panel) to demonstrate equivalent loading.