Figure 2
From: Keratin and S100 calcium-binding proteins are major constituents of the bovine teat canal lining
Immunofluorescence of bovine teat canal tissue. Teat canal cryosections were stained using indirect immunofluorescence for (A) S100A7/A7-like, (B) S100A8, (C) S100A9 and (D) S100A12. Serial sections were probed with anti-S100 protein antibodies and the signal detected with Alexa Fluor labelled secondary antibodies (red signal). Each section was counterstained with DAPI (blue signal). Equivalent sections probed with non-specific IgG are shown as inserts on the upper right hand corner. All micrographs were exposed for the same length of time as the negative controls. L: lumen, E: epidermis, D: dermis, M: Marksäulchen, S: stratum spinosum. Scale bars in all panels are 100 μm.