Figure 3

Representative flow cytometry dot plots and peripheral blood Treg-cell population. Peripheral blood samples were collected from the indicated pigs at 0, 24, and 144 h after F4⁺ ETEC/VTEC/EPEC challenge. Left, representative flow cytometry dot plots. Right, flow cytometry was used to determine the percentages of (A) Foxp3⁺, (B) IL-10⁺, (C) Foxp3⁻IL10⁺ and Foxp3⁺IL10⁺ Treg cells within the peripheral blood CD4⁺ T-cell population in the indicated pigs. Data are presented as the mean ± SEM for each time point (n = 8 per group). *P < 0.05; **P < 0.01; Tukey’s test.