A combinational approach of multilocus sequence typing and other molecular typing methods in unravelling the epidemiology of Erysipelothrix rhusiopathiae strains from poultry and mammals

Table 1 Primer sets used to amplify and sequence E. rhusiopathiae housekeeping genes and genetic diversity of gene loci used for MLST analysis

Gene	Primer sequence [5′-3′]	Location within gene^a	Amplicon size PCR (bp)	Allele size MLST (bp)	Mean GC content	No. of alleles	No. of variable sites (%)	d _N /d _Sratio	Simpsons index of diversity (D)
gpsA	fw: AGTTATGATGTGGGGACG	75-745	671	540	38.3%	9	11 (2.0)	0.3610	0.608
gpsA	rv: TAGCTGTAACGACGAGATCG	75-745	671	540	38.3%	9	11 (2.0)	0.3610	0.608
recA	fw: TTCGGTAGAATAATCTCGCG	38-913	876	640	39.7%	9	13 (1.6)	0.0207	0.775
recA	rv: TGCTATTAGTTCAGGGTCG	38-913	876	640	39.7%	9	13 (1.6)	0.0207	0.775
purA	fw: GATGTTTATGAGGAAGCGC	331-1066	736	611	39.1%	14	11 (1.8)	0.5690	0.764
purA	rv: AACGCATTGATTGTTGCCC	331-1066	736	611	39.1%	14	11 (1.8)	0.5690	0.764
pta	fw: TGCTGCAGTACGTTTAGC	90-793	704	544	36.6%	9	12 (2.2)	0.0419	0.579
pta	rv: AGACACGTGCATTACCTG	90-793	704	544	36.6%	9	12 (2.2)	0.0419	0.579
prsA	fw: ACAAGTTCACCAGTAAGTG	190-952	763	575	39.3%	6	4 (0.7)	0.0654	0.657
prsA	rv: AGAGTGTACTTACAGGAGT	190-952	763	575	39.3%	6	4 (0.7)	0.0654	0.657
galK	fw: TATTCCTAATGGAGCGGG	357-1041	685	554	36.8%	13	10 (1.8)	0.0766	0.740
galK	rv: AATCGCAATCGCACATCC	357-1041	685	554	36.8%	13	10 (1.8)	0.0766	0.740
ldhA	fw: AACGGATATGAAGCTGTTGCC	136-783	648	531	40.3%	11	14 (2.6)	0.3390	0.681
ldhA	rv: AAGAACATCCAGTCCAACAGC	136-783	648	531	40.3%	11	14 (2.6)	0.3390	0.681

^aAs referred to E. rhusiopathiae ATCC 19414^T (Acc.-No. NZ_ACLK02000001 - NZ_ACLK02000004).

ISSN: 1297-9716