Proteomic analysis of purified turkey adenovirus 3 virions

Table 4 Cellular proteins associated with purified TAdV-3 virions after proteinase K treatment

	LC-MS/MS
Protein name	Mass (kDa) ^a	No. of peptides	Mascot score ^b	Sequence coverage (%) ^c	Protein function	Reported in other viruses
Protein PML isoform X6	48.4	8	141	10	Innate immunity	-
Collagen alpha-1 (VI) chain	110	17	101	7	Cell adhesion	-
Vitronectin	52.2	4	100	5	Cell adhesion	SIV²¹, KSHV²⁹
Hemoglobin subunit alpha –A like	15.5	5	99	24	Innate immunity	Corona ²⁶ , Influenza ²⁰
Collagen alpha-3 (VI) chain	340	9	92	10	Cell adhesion	-
Collagen alpha-2 (VI) chain	110	5	91	5	Cell adhesion	Influenza²⁰
Ferritin	17.1	4	90	20	Virus replication	HCV ²⁴ , sHEV ²⁵
Elongation factor 1-alpha	47.6	2	69	3	Virus replication	HIV ¹³
Hemoglobin subunit-beta like	16.3	8	66	27	Innate immunity	CSFV ⁴⁵
Tyrosine protein phosphatase	68	2	63	2	Cell division	-
Antimicrobial peptide THP-2	7.6	7	64	48	Innate immunity	-
Splicing factor U2AF	28.7	5	60	9	Splicing factor	KSHV ²⁹
Serine/arginine splicing factor 5α	30.1	1	43	3	Splicing factor	Influenza ²⁰
TAR DNA binding protein 43	45.0	3	40	2	Transcription	HSV ¹⁴ ,RSV ²³
L-amino acid oxidase	59.08	4	40	7	Flavoprotein	-
Gallinacin-2	7.6	4	39	26	Innate immunity	-
Tubulin alpha-1A	50.9	4	32	4	Cytoskeleton	HIV ¹³ , Influenza ¹¹ , ASFV ²⁸
Actin	42.2	3	32	5	Cytoskeleton	HIV ¹³ , Influenza ¹¹ , ASFV ²⁸

Protein subsets identified by LC-MS/MS with/without protease treatment are shown in bold black.
^aTheoritical molecular mass.
^bA Mascot score ≥30 is significant (p < 0.05).
^cSequence coverage is based on peptides with an unique sequence.

ISSN: 1297-9716