Neuroanatomical distribution and morphological patterns of PrP
accumulation in the brain of ICR mice infected with sheep-passaged L-BSE, at second passage.
A The 5 coronal brain sections are as follows: 1. frontal cortex, 2. septal level, 3. hippocampus and thalamic level, 4. midbrain, and 5. medulla with cerebellum. PrP-plaque deposits are abundant throughout the cerebral gray matter (1 to 4) and hippocampus (3 and 4). Granular and aggregated PrPSc accumulated in the septal nuclei (2), hypothalamus (3), and temporal cortex (4). Scale bar = 1 mm. B Histopathology and PrPSc immunohistochemical analysis with mAb SAF84 revealed plentiful florid plaques accompanied by granular and aggregated PrPSc deposits in the hippocampus. Insets show high-magnification images of a florid plaque. Scale bar = 100 μm, scale bar in inset = 25 μm.