IL-17A increases the secretion of CXCL8 by PS cells upon stimulation with
strains 1303 and P4.
A-D: PS cells were incubated for 3 hours with strain E. coli 1303 or P4 at an MOI of 1. Cells were then washed twice with HBSS and medium with gentamycin was added with or without 100 ng/mL IL-17A. Response was analysed in terms of gene expression (CCL20: A, CXCL8: B, TAP: C) or CXCL8 secretion by ELISA (D) after beginning of the experiment. Data presented are mean values and SD obtained from 3 independent experiments. Experiments were performed at passages 8, 26 and 30. E-F: primary mammary epithelial cells from two cows were incubated for 3 hours with strains E. coli 1303 and P4 at an MOI of 1. Cells were then washed twice with HBSS and medium with gentamycin was added with or without 100 ng/mL IL-17A. CXCL8 secretion was measured by ELISA 24 hpi. Data presented are mean values obtained with cells stimulated in triplicates. * statistical significance (P < 0.05) calculated using exact permutation tests after global comparison using a Kruskal and Wallis test.