IL-17A effect on the response of PS cells to Pam3-CSK4. PS cells were incubated for 5 h with 100 ng/mL of Pam3-CSK4 in the presence or not of 100 ng/mL IL-17A. Response was analyzed in terms CXCL8 secretion (A) by ELISA or expression of CCL20 (B) and CXCL8 (C) by RT-qPCR. Data presented are mean values and SD obtained from 3 independent experiments. Experiments were performed at passages 15, 16 and 24. * statistical significance (P < 0.05) calculated using exact permutation tests after global comparison using a Kruskal and Wallis test.