Figure 3

Innate immune response of PS cells to different purified bacterial agonists. PS cells were incubated for 5 h with the indicated concentrations of different purified agonists. Response was analyzed in terms of expression of CCL20 (A), CXCL8 (B), TAP (C), LAP (D) genes by RT-qPCR or CXCL8 secretion by ELISA (E,F). Data presented are mean values and SD obtained from 3 independent experiments. Experiments were performed at passages 11, 12 and 24. PS cells were incubated for 5 hours in GM medium with different agonists at the indicated concentrations. (F) PS cells were incubated with ultrapure LPS (LPS) at 10 ng/mL in the presence of either 10% fetal calf serum, 5 μg/mL CD14 or 5 μg/mL LBP. Stimulation was also performed in whole milk instead of GM medium. * statistical significance (P < 0.05) of stimulated versus unstimulated PS cells using exact permutation tests after global comparison using a Kruskal and Wallis test.