Figure 2

Characterization of PS cells properties by flow cytometry and RT-PCR. PS cells, at passage 14, were labelled with antibodies against CD45 (A), cytokeratin 14 (B), cytokeratin 18 (C), TLR2 (D) and CD14 (E). Controls were performed either by omitting the primary antibody (A, B, C) or using unlabelled cells (D, E). As a control for CD45 labelling, bovine PBMC were labelled with the anti-CD45 antibody (F). Labelled cells are depicted as red curves while controls are represented as blue curves. (G-H) RNA was extracted from unstimulated PS cells and converted to cDNA. cDNAs for each of the indicated receptors were amplified by PCR and products were separated by agarose gel electrophoresis. MW: 50 bp molecular weight ladder.