Figure 7
From: Invasion and persistence of Mycoplasma bovis in embryonic calf turbinate cells
Fluorescence microscopy of the gentamicin protection assay with permeabilized and not permeabilized cells using antibodies directed against M. bovis . MOI of 10; 54 hours post infection. Panel A: Permeabilized PECT cells without gentamicin treatment infected with M. bovis JF4278. Mycoplasmas are visible intra- and extracellularly. Panel B: Non permeabilized PECT cells without gentamicin treatment infected with M. bovis JF4278. Only extracellular mycoplasmas are visible. Panel C: Permeabilized PECT cells with gentamicin treatment infected with M. bovis JF4278. Only intracellular mycoplasmas are visible. Panel D: Not permeabilized PECT cells with gentamicin treatment infected with M. bovis JF4278. No mycoplasmas are visible. Nuclei are stained in blue, F-actin is stained in red, mycoplasmas are stained in green. Merged images. Magnification 600 ×.