Antigen
|
Clone
|
Isotype
|
Fluorochrome
|
Labeling strategy
|
Source of primary Ab
|
---|
Phenotyping of moDCs
|
CD1
|
76-7-4
|
IgG2a
|
Alexa647
|
secondary antibodya
|
in housej
|
CD14
|
CAM36A
|
IgG1
|
BV421
|
secondary antibodyb
|
VMRD
|
CD80/86
|
CD152/Fc chimera
|
IgG2a
|
Alexa647
|
secondary antibodya
|
Sigma-Aldrich
|
CD163
|
2A10/11
|
IgG1
|
PE
|
directly conjugated
|
AbD Serotec
|
CD169
|
3B11/11
|
IgG1
|
BV421
|
secondary antibodyb
|
AbD Serotec
|
CD172a
|
74-22-15A
|
IgG2b
|
Alexa488
|
secondary antibodyc
|
in housej
|
SLA-DR
|
MSA3
|
IgG2a
|
Alexa647
|
secondary antibodya
|
in housek
|
Assessment of moDC infection rate
|
CD172a
|
74-22-15A
|
IgG2b
|
PE
|
secondary antibodyd
|
in housej
|
PRRSV-N
|
P10/b1
|
IgG1
|
Alexa488
|
directly conjugatede
|
in housel
|
CD163, CD169, SLA-DR and CD80/86 expression on infected moDCs
|
CD172a
|
74-22-15A
|
IgG2b
|
Alexa647
|
secondary antibodyf
|
in housej
|
SLA-DR
|
MSA3
|
IgG2a
|
PE
|
directly conjugatedg
|
in housek
|
CD80/86
|
hCTLA4/Fc chimera
|
IgG2a
|
BV421
|
two step biotin-streptavidinh
|
Sigma-Aldrich
|
CD163
|
2A10/11
|
IgG1
|
PE
|
directly conjugated
|
AbD Serotec
|
CD169
|
3B11/11
|
IgG1
|
BV421
|
two step biotin-streptavidini
|
AbD Serotec
|
PRRSV-N
|
P10/b1
|
IgG1
|
Alexa488
|
directly conjugatede
|
in housel
|
-
aGoat anti-Mouse IgG2a-Alexa647, Life Technologies.
-
bRat anti-Mouse IgG1-BV421, clone RMG1-1, BioLegend.
-
cGoat anti-Mouse IgG2b-Alexa488, Life Technologies.
-
dGoat anti-Mouse IgG2b-PE, Southern Biotech.
-
eAlexa Fluor® 488 Protein Labeling Kit, Life Technologies.
-
fGoat anti-Mouse IgG2b-Alexa647, Life Technologies.
-
gLightning-Link™ RPE Conjugation Kit, Innova Biosciences.
-
hGoat anti-Mouse IgG2a-biot., Southern Biotech; Streptavidin-BV421, BioLegend.
-
iGoat anti-Mouse IgG1-biot., Southern Biotech; Streptavidin-BV421, BioLegend.
-
jPescovitz et al. [27].
-
kHammerberg and Schurig [28].
-
lWeiland et al. [29].