Figure 2

Frequency of PRRSV-infected moDCs. MoDCs were either mock-infected or infected with a PRRSV-2 or a PRRSV-1 strain and either cultivated for the indicated periods of time or infected with different MOI rates. (A) Contour plots show fluorescence intensity of PRRSV-nucleoprotein (PRRSV-N) staining versus Live/Dead discrimination dye of mock-infected (mock), PRRSV-2 and PRRSV-1 infected CD172a⁺ gated (not shown) moDCs after 24 hpi. Coloured gates indicate the following cell subpopulations: non-infected and live moDCs (green gate), infected and live moDCs (red gate) and infected and dead moDCs (blue gate). An MOI of 0.1 was used for both PRRSV-genotypes. (B) Scatter diagrams show the frequency of either PRRSV-1 infected live or PRRSV-1 infected dead moDCs (y-axis) after 0, 12, 24 or 48 hpi (x-axis). An MOI of 0.1 was used for infection. (C) Frequencies of either live (L) or dead (D) infected moDCs for different MOI rates (x-axis; separated by dashed lines) in PRRSV-2 (left scatter diagram) and PRRSV-1 infected moDCs (right scatter diagram) after 24 hpi are shown. (B + C) Each symbol represents data of moDCs derived from one individual animal (B: n = 4; C: n = 5). Coloured bars indicate mean values.