Figure 5

IFN-γ/TNF-α/IL-2 expression levels and CD8α/CD27 expression of cytokine-producing CD4 ⁺ T cells. (A + B) Fluorescence intensity of IFN-γ, TNF-α and IL-2 expression within single- (dark grey), double- (orange) and triple- (red) cytokine-producing CD4⁺ T cells. (A) Histograms show exemplary data of animal #9 at six weeks post primary infection. Numbers indicate median fluorescence intensity (MFI) for each subset and the respective cytokine. (B) MFI of IFN-γ, TNF-α and IL-2 expression within single- (dark grey), double- (orange) and triple- (red) cytokine-producing CD4⁺ T cells isolated at six weeks post primary infection. Data of all six infected animals is shown by individual symbols. The mean is indicated by the black bar. (C) CD8α and CD27 expression on total CD4⁺ T cells (contour plot in the upper left and light grey dots in dot plots) and CD4⁺ T cells producing a single cytokine (dark grey dots, bottom), two cytokines (orange dots, middle) or three cytokines (red dots, top). Representative raw data of animal #9, six weeks post primary infection, is shown.