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Figure 2 | Veterinary Research

Figure 2

From: Gene expression profiling of porcine mammary epithelial cells after challenge with Escherichia coli and Staphylococcus aureus in vitro

Figure 2

Validation of cell types in PMEC cultures. (A) Phase contrast micrograph of a confluent PMEC monolayer grown on collagen-coated tissue culture dishes demonstrating typical epithelial cobblestone morphology (bar = 100 μm). (B) Dominant luminal mammary epithelial cells were stained with anti-cytokeratin-18 antibody (anti-Cy18, green fluorescence; nuclei, DAPI, blue fluorescence). (C) Sporadically found myoepithelial cells were stained with anti-smooth muscle actin antibody (anti-Actin, green fluorescence; nuclei, DAPI, blue fluorescence).

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Veterinary Research

ISSN: 1297-9716