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Figure 6 | Veterinary Research

Figure 6

From: Evaluation of biological safety in vitro and immunogenicity in vivo of recombinant Escherichia coli Shiga toxoids as candidate vaccines in cattle

Figure 6

Gene transcription in bovine iIEL and surface marker expression on bovine MDM incubated for 6 h with rStx WT or rStx mut . (A) Relative amounts of gene transcripts for IL-4 harboured by PHA-P-stimulated (2.5 mg/mL) bovine iIEL normalized to the transcription of the housekeeping gene GAPDH. Vector control cultures were used as reference and set to 100% (dashed line). (B) Expression (i.e., mean fluorescence intensity; MFI) of CD14 on bovine CD77+ MDM relative to vector control cultures defined as 1.0 (dashed line). Data is depicted as means ± standard deviations from 5 independent experiments each. Statistical analysis was performed as described in legend to Figure 2. Significance levels were defined as p ≤ 0.01 [**], and p ≤ 0.05 [*].

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