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Figure 4 | Veterinary Research

Figure 4

From: Evaluation of biological safety in vitro and immunogenicity in vivo of recombinant Escherichia coli Shiga toxoids as candidate vaccines in cattle

Figure 4

Effect of recombinant Shiga toxins and toxoids on CD77 expression by bovine CD8β + PBMC. Cells were incubated in culture medium containing 5 μg/mL PHA-P and rStxWT or rStxmut as indicated. After four days of incubation cells were submitted to immunolabelling and flow cytometry analysis. Percentages of CD8β+ PBMC co-expressing CD77 (events in the upper right quadrant) are given in the upper right corner of the dot plots. PBMC incubated in the presence of PHA-P and vector control were used as control (upper graph).

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