Figure 2

Proportion of CD21 ⁺ , CD4 ⁺ , CD8β ⁺ , and γδT ⁺ cells in cultures of PHA-P stimulated bovine PBMC after incubation with recombinant Shiga toxins and toxoids. Results are shown relative to data obtained from cultures incubated in the presence of the vector control (control cultures; defined as 1.0, indicated by the dashed line). Data is depicted as means ± standard deviations of 3 to 6 repetitive experiments as indicated. ANOVA was performed (1) comparing non-normalized data with the values from control cultures (asterisks above bars) and (2) comparing values of normalized data obtained after incubation with rStx1_WT versus rStx1_mut, rStx2_WT versus rStx2_mut, rStx1_WT versus rStx2_WT, and rStx1_mut versus rStx2_mut (asterisks above brackets). Significance levels were defined as p ≤ 0.001 [***], p ≤ 0.01 [**], and p ≤ 0.05 [*].