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Figure 1 | Veterinary Research

Figure 1

From: The immunoglobulin M-degrading enzyme of Streptococcus suis, Ide Ssuis , is involved in complement evasion

Figure 1

Ide Ssuis cleaves the heavy chain of porcine IgM. (A) αIg light chain and αIgM Western blot analysis of purified porcine IgM after incubation +/− rIde Ssuis . Samples were separated in an SDS-gel under reducing conditions. The marker bands are shown on the left side (sizes in kDa). (B) The IgM-cleavage product highlighted in red was characterized by N-terminal Edman-sequencing. The identified sequence is shown in red and corresponds to the constant domain 3 of the IgM heavy chain. The putative C1q binding motif is marked in blue. Domains C2 and C4 are underlined. The obtained sequence was taken directly from the NCBI database. (C) Illustration of an IgM-monomer with the indicated cleavage site and the location of the putative C1q-binding motif.

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Veterinary Research

ISSN: 1297-9716