Table 1 Primers used for qPCR experiments
From: Mycoplasma iowae: relationships among oxygen, virulence, and protection from oxidative stress
Primer Name | Target | Sequence (5′-3′) | Tm (°C) |
|---|---|---|---|
MI16Sleft(qPCR) | 16S rRNA gene | CGCAAGACTCACGAGCTTAT | 54.6 |
MI16Sright(qPCR) | GGTACAAACTGTCGCAAACC | 54.4 | |
MIcards1left(qPCR) | cards1 (P271_571) | TGGGTAGAAGCACAGACGTT | 56.1 |
MIcards1right(qPCR) | ACTCATCTGCATCTGGGTCA | 55.8 | |
MIglpFleft(qPCR) | glpF (P271_673) | ATCTAGCATGATGGGTGGCG | 57.3 |
MIglpFright(qPCR) | TGTCCAAACATTGCTCCTGT | 54.7 | |
MIkatEleft(qPCR) | katE (P271_534) | CGTGTAGTTCATCGAAAAGGTG | 54.6 |
MIkatEright(qPCR) | CTTCCAGCTTCACCACCAAC | 56.3 | |
MIsodleft(qPCR) | sodA (P271_491) | ACACAAAGCATCACCAAGCT | 55.2 |
MIsodright(qPCR) | TGATTGTGATGACCTCCACCA | 56.1 |