Figure 4
Effect of glutamine starvation on GSH levels, MDA concentration and PCV2 replication. After 24 h of PCV2 infection in complete medium, fresh glutamine-free medium with various concentrations of glutamine and BSO was added to replace the original medium. The cells were cultured for another 48 h before the determining of GSH levels (A and C), MDA concentration (B), the number of PCV2 log 10 DNA copies per 106 cells (D), and the proportion of PCV2 infected cells (E and F). Values are shown as mean ± SD from three independent experiments. Asterisks indicate groups statistically significantly different from control by a 1-way ANOVA followed by a least-significant difference test (*P < 0.05, **P < 0.01).