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Figure 2 | Veterinary Research

Figure 2

From: Glutamine starvation enhances PCV2 replication via the phosphorylation of p38 MAPK, as promoted by reducing glutathione levels

Figure 2

Effects of different concentrations of glutamine on PCV2 replication. PK-15 cells were infected with PCV2 at an MOI of 1 and incubated in the complete medium for 24 h. Subsequently, PK-15 cells were grown in medium containing various concentrations of glutamine. After 48 h, the PK-15 cells were harvested to determine the number of PCV2 log 10 DNA copies per 106 cells (A) and the relative proportion of PCV2-infected cells (B and C) by real-time PCR and immunofluorescence microscopy, respectively. Values shown are means ± SD mean from three independent experiments. Asterisks indicate groups statistically significantly different from control by a 1-way ANOVA followed by least-significant difference test (**P < 0.01).

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