Figure 2

Amplication, cloning and expression of FhTLM. (A) 1.5% Agarose gel electrophoresis of amplified PCR fragments from 5’ RACE (left panel) and 3’ RACE (right panel). 5'-RACE product of 1830 bp and 3'-RACE product of 1994 bp were obtained from nested PCR, M, 1 Kb DNA ladder. (B) Coomassie stained SDS PAGE of protein expression from transformed Rosetta BL21 induced by 1 mM IPTG. Image represents native conditions. M; Protein marker, Lane 1; Supernatant uninduced Rosetta BL21, Lane2; Supernatant induced Rosetta BL21, Lane 3; Elution buffer 20 mM imidazole, Lane 4; Elution buffer 40 mM imidazole, Lane 5; Elution buffer 60 mM imidazole, Lane 6; Elution buffer 80 mM imidazole. (C) Organisation of the FhTLM gene depicting regions of the ORF (blue), exons (dashed lines), and UTRs (white) and their size in bases.