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Table 2 Characterization of anti-SBV mAbs by ELISA, neutralization test, and western blot

From: A novel panel of monoclonal antibodies against Schmallenberg virus nucleoprotein and glycoprotein Gc allows specific orthobunyavirus detection and reveals antigenic differences

MAbs

ELISA

VNT

Western blot (kDa)

    
   

Schmallenberg virus

DOUV

SIMV

SATV

SHAV

SABOV

AKAV

AINOV

OROV

 
   

BH80/11-4

BH619/12-3

BH652/12

D512/12

D495/12-1

D495/12-2

        

Specifity

4E12

+

<1/2

~25

~25

~25

~25

~25

~25

~25

~25

~25

~25

~25

~25

-

-

nucleocapsid protein

1H4

+

<1/2

~25

~25

~25

~25

~25

~25

~25

~25

~25

~25

~25

~25

-

~25

nucleocapsid protein

3H3

+

<1/2

~25

~25

~25

~25

~25

~25

~25

~25

~25

~25

~25

~25

-

-

nucleocapsid protein

3E3

+

<1/2

~25

~25

~25

~25

~25

~25

~25

~25

~25

~25

-

-

-

-

nucleocapsid protein

3H9

+

<1/2

-

-

-

-

-

-

 

-

-

-

-

   

nucleocapsid protein

3A11

+

<1/2

-

-

-

-

-

-

 

-

-

-

-

   

nucleocapsid protein

2G10

+

<1/2

~110

~110

~110

~110

~110

~110

        

glycoprotein Gc

4D9

+

pos

~110

~110

~110

~110

~110

~110

        

glycoprotein Gc

2H11

+

<1/2

~110

~110

~110

~110

~110

~110

~110

-

-

-

-

   

glycoprotein Gc

4E5

+

pos

~110

~110

~110

~110

~110

~110

        

glycoprotein Gc

5 F8

+

1/8

~110

-

-

-

-

-

        

glycoprotein Gc

1C11

+

1/16

~110

~110

~110

~110

~110

~110

-

-

~110

-

-

   

glycoprotein Gc

1 F4

+

1/4

~110

~110

~110

~110

~110

~110

-

-

-

-

-

   

glycoprotein Gc

4B6

+

1/8

~110

~110

~110

~110

~110

~110

        

glycoprotein Gc

1C1

+

1/16

~110

~110

~110

~110

~110

~110

 

-

~110

-

-

   

glycoprotein Gc

3A5

+

<1/2

~110

~110

~110

~110

~110

~110

 

-

~110

-

-

   

glycoprotein Gc

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Veterinary Research

ISSN: 1297-9716