Figure 3
From: PCV2 vaccination induces IFN-γ/TNF-α co-producing T cells with a potential role in protection
PCV2-specific IgM/IgG antibodies and coincidence with viremia. Serum samples were analysed for PCV2-specific antibodies by a commercially available ELISA kit (INGEZIM Circovirus IgG/IgM, Ingenasa, Madrid, Spain). A) The heatmap shows the detection of PCV2-specific IgM and IgG antibodies for all animals of the different study groups (CO = control, VA = vaccinated, IN = infected, VI = vaccinated & infected). Light grey fields represent samples positive for IgG. Dark grey fields indicate the detection of IgM antibodies. Black fields illustrate samples containing both IgM and IgG antibodies. PCV2 infection was performed 24 days post vaccination. Corresponding days post infection are indicated at the bottom of the table. Occurrence of PCV2 DNA in individual samples is marked by X. X indicates serum samples with < 104 PCV2 copies/mL, XX stands for > 104 PCV2 copies/mL. B) Raw data of the INGEZIM Circovirus IgG/IgM ELISA is depicted. OD-values, measured at 450 nm, of IgM (left) and IgG antibodies (right) for individual animals and all sampling points are shown. The red lines indicate the cut-off OD = 1.108 for IgM and OD = 0.821 for IgG, respectively.