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Table 1 Results of virus isolation, RT-PCR (OPF swabs only), antibody detection and detection of FMD clinical signs

From: Quantification of transmission of foot-and-mouth disease virus caused by an environment contaminated with secretions and excretions from infected calves

Exp a

Calf ID

I:C b

Group

FMDV detection by virus isolation in OPF swabs (in log10 titres),blood(v), urine(u) and faeces(f) samples and by RT-PCR (OPF swabs only, in bold or as ≡)

Antibody detection

Clin c Inf d

    

days post infection of the inoculated calves

NS-ELISA

VNT

 
    

1

2

3

4

5

6

7

8

9

10

11

12

13

14

15

16

17

18

19

20

    

DC

3643

I

 

≡e

2.2 f

≡,vg

2.6,v,uh

3.8,v,u

2.0,u

-

-

-

-

-

-

-

-

      

+

+

Yes

Yes

 

3644

C

 

-

-

-

-

1.5

-,v,u

2.1,v

1.4,v

≡

1.7,u

-

-

1.2

≡

      

+

+

Yes

Yes

 

3645

I

 

-

-

≡,v

2.5,v,fi

2.6,v

0.9,u

1.2,u

0.7

0.7

-

-

-

-

-

      

+

+

Yes

Yes

 

3646

C

 

-

-

-

-

-

≡

3.8

4.3,v

3.0,v,u

3.0,u

-,u

0.4

-

-

      

+

+

Yes

Yes

 

3647

I

 

-

≡

-

-

-

-

-

-

-

-

-

-

-

-,u

      

+

+

No

No

 

3648

C

 

-

-

-

-

-

-

-

-

-

-

-

-

-

-

      

-

-

No

No

 

3649

I

 

-

-

0.7,v

2.3,v,u

3.4,v

0.9,u

-,u

-,u

-

-

-

-

-

-

      

+

+

Yes

Yes

 

3650

C

 

-

-

-

-

-

2.3,v,u

1.6,v,u,f

0.4,u,f

≡,u

0.9

0.4

-,u

-

-

      

+

+

Yes

Yes

 

3651

I

 

-

-

1.4

≡

4.2

1.7

0.4

-

-

-

-

-

-

-

      

+

+

Yes

Yes

 

3652

C

 

-

-

-

-

-

-

-

0.4

-

-

-

-

-

-

      

+

+

No

Yes

IC

3653

I

A

-

-

n.tj

1.7,v,u

3.4,v

2.1,u

              

-

-

Yes

Yes

 

3654

I

A

-

≡

6.0,v

4.6,v

3.7,v,f

1.7

              

-

-

Yes

Yes

 

3657

C1

A

  

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

No

No

 

3658

C1

A

  

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

No

No

 

3661

C2

A

     

-

-

-

-

-

3.7,v

≡,v,u

≡,v,u

2.4

≡,u

≡

≡

-

-

-

+

+

Yes

Yes

 

3662

C2

A

     

-

-

-

-

2.0

≡

≡

≡,v

1.3,v

3.8,v,u

3.5,v,u

3.6,u

≡

-

≡

+

+

Yes

Yes

 

3655

I

B

-

-

2.1

5.2,v

4.9,v,u

2.6,u

              

-

-

No

Yes

 

3656

I

B

-

0.4

≡,v

3.2,v,u

5.0,v,u,f

3.5,u

              

-

-

No

Yes

 

3659

C1

B

  

-

-

1.9

2.0

3.6,v,f

4.9,v,u

4.1,v,u

3.4,u

-

1.2

-

-

-

-

-

-

-

-

+

+

Yes

Yes

 

3660

C1

B

  

-

-

-

-

0.9

1.3,u

0.7

1.0

-

-

-

-

-

-

-

-

-

-

-

-

Yes

Yes

 

3663

C2

B

     

-

-

-

-

-

-

-

2.4

0.7

1.6

3.3,u

2.3,u

≡

-

-

+

+

Yes

Yes

 

3664

C2

B

     

-

0.4

1.0

1.9

3.0,v

5.2,v

≡,v

2.6

0.4

≡

-

-

-

-

-

+

+

Yes

Yes

  1. aExp=experiment: DC=direct contact, IC=indirect contact; bI=inoculated, C=contact animal; cClin=clinical signs; dInf=infectious; eresults of virus isolation (VI) and RT-PCR of oral swab sample: - = VI and RT-PCR negative, ≡ = VI negative and RT-PCR positive; foral swab sample scored positive for FMDV by VI (log10 pfu/mL), RT-PCR positive samples are indicated in bold; gv=viraemia: blood sample scored positive for FMDV by VI; hu=urine sample scored positive for FMDV by VI; if=faeces sample scored positive for FMDV by VI; jn.t.=not tested. Table in which the PCR positive samples are indicated (thus either the ≡ or VI titres in bold) in an effort to help with the editorial of this table.
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Veterinary Research

ISSN: 1297-9716