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Figure 4 | Veterinary Research

Figure 4

From: Eimeria bovis-triggered neutrophil extracellular trap formation is CD11b-, ERK 1/2-, p38 MAP kinase- and SOCE-dependent

Figure 4

E. bovis sporozoite-induced NETosis in bovine PMN is a NADPH oxidase-, NE- MPO-dependent process. (A-C) NADPH oxidase, NE and MPO enzymatic activities in E. bovis sporozoite-exposed PMN. PMN were exposed to E. bovis sporozoites and enzymatic activities of NADPH oxidase (A), NE (B) and MPO (C) were measured using via DCFH-DA oxidation, NE-chromogenic substrate analysis and using Amplex red reagent, respectively. (D) Sporozoite-triggered NET formation after inhibition of NADPH oxidase (via DPI), NE (via CMK) and MPO (via ABAH). For inhibition and maximum DNA release controls, DNase I and Triton X treatments were used, respectively. Stimulation with zymosan served as a positive control (1 mg/mL) and PMN in plain medium as a negative control.

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