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Figure 3 | Veterinary Research

Figure 3

From: Structural insight in the inhibition of adherence of F4 fimbriae producing enterotoxigenic Escherichia coli by llama single domain antibodies

Figure 3

VHH inhibition of the adherence of F4 fimbriated E. coli to piglet enterocytes in vitro. Quantitative analysis of bacterial adherence to piglet enterocytes of strains expressing F4ab (A), F4ac (B) and F4ad (C) fimbriae. A two-fold dilution series of monomeric VHHs was added to 4 × 108 F4+ cells and incubated during one hour with an average of 50 villi. Villi were examined by phase-contrast microscopy at a magnification of 600× and the number of bacterial cells adhering to 250 μm villi length (5 repeated reads for each test sample) were counted and plotted as a percentage of wild type binding.

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Veterinary Research

ISSN: 1297-9716