Comparative 2DE analysis of
proteins and immunoblotting profiles of specific reactions. (A) A total of 55 immunoreactive spots of antigen that responded to antisera from cattle after 3, 7 and 10 weeks of challenge with B. abortus were selected. A total of 19, 10, and 4 immunoreactive spots of antigen responded to antisera at 2 time-points: (B) after 3 and 7 weeks of challenge, (C) after 3 and 10 weeks, and (D) after 7 and 10 weeks; these spots were selected and labeled on the 2DE gel. The numbers represent the serial numbers of the immunoreactive proteins in immunoblot analyses.