Figure 4

Immunoblotting profile of B. abortus proteins responded with B. abortus -infected bovine antisera excluding non-specific proteins. Immunoblotting analyses were performed with antisera from cattle after 3, 7, and 10 weeks of challenge with B. abortus. After excluding non-specific reactions, a total of 101 (A), 84 (B), and 78 (C) immunoreactive dots, as well as 55 protein spots that reacted with antisera at all 3 stages (D , E, and F), were selected and labeled. The numbers represent the serial numbers of the immunoreactive proteins in immunoblot analyses.