2DE profile of
proteins and immunoblotting with antisera from
-infected cattle. (A) 2DE profile of proteins from B. abortus detected on silver-stained 2DE gels within the pI range 4–7. Immunoblotting analyses were performed with antisera from cattle after 3 (B), 7 (C), and 10 weeks (D) of challenge with B. abortus. Three replicates of 2DE analysis were performed in the independent experiments.