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Table 3 Pathogenicity of H9N2 AIV to SPF chickens a

From: Amino acid substitutions in the neuraminidase protein of an H9N2 avian influenza virus affect its airborne transmission in chickens

Virus Lethality Virus titers in trachea (log 10 EID 50 /gram ± SD) Virus titers in lung (log 10 EID 50 /gram ± SD) Seroconversion (HI titers)
rSD01 None 5.83 ± 0.29 5.33 ± 0.34 64, 128
r01/NASS None 3.72 ± 0.35* 3.61 ± 0.26* 64, 64
r01/NAHB None 5.67 ± 0.34 5.21 ± 0.18 128, 64
r01/NA381 None 4.17 ± 0.29* 3.56 ± 0.10* 128, 64
  1. aSPF chickens (n = 5) were inoculated intranasally 107 EID50 of each virus. Clarified homogenates of tracheas and lungs from three animals collected on 5 dpi were titrated for virus infectivity in SPF embryonated chicken eggs from initial dilutions of 1:10. Virus titers were expressed as mean log10EID50/g wet tissues ± standard deviation (SD). Two chickens were observed for signs of lethality, and seroconversion was confirmed by hemagglutination inhibition (HI) assay on 14 dpi. *P < 0.05 compared with rSD01.