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Figure 7 | Veterinary Research

Figure 7

From: The early intestinal immune response in experimental neonatal ovine cryptosporidiosis is characterized by an increased frequency of perforin expressing NCR1+ NK cells and by NCR1 CD8+ cell recruitment

Figure 7

Perforin+ lymphocytes among CD8+ lymphocytes in the gut . (A) Cells extracted from gut tissues were double labelled with anti NCR1 and CD8 mAbs then fixed and permeabilized and labelled with an anti perforin mAb. The plot shows (example of jejunum at 6 dpi) the gating of the lymphocyte populations analyzed. (B) Lymphocytes from an inoculated lamb (3 dpi) and its control were gated either on the CD8+/NCR1+ or the CD8tot/NCR1- populations then analyzed for the perforin content. The red line limited histogram corresponds to the inoculated lamb (In), the black line limited histogram to its matched control (Co) and the gray filled histogram to the isotype control of the perforin mAb (iso) and the mean fluorescence intensity (MFI) is indicated for each histogram. (C) The analysis of the perforin expression by CD8+ lymphocyte sub-populations (gates shown on Figure 7A) was performed in 3 control and 4 age-matched 6 dpi inoculated lambs. The comparisons were made with the Mann–Whitney (control/inoculated) and Wilcoxon tests (paired data). Statistically significant differences are indicated with ** p < 0.01 and *** p < 0.001.

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