Table 2 Primers used for H7-FliC mutagenesis
SDM | Orientation | Oligonucleotide sequence (5’ – 3’) |
|---|---|---|
L500A/I504A | F | CCCGCTTGCTGCCGCGGACGACGCAGCCAGCTCCATCGAC |
| Â | R | GTCGATGGAGCTGGCTGCGTCGTCCGCGGCAGCAAGCGGG |
Q89A | F | CAACAACAACTTAGCGCGTATTCGTGAAC |
| Â | R | GTTCACGAATACGCGCTAAGTTGTTGTTG |
Q89D | F | CAACAACAACTTAGACCGTATTCGTGAAC |
| Â | R | GTTCACGAATACGGTCTAAGTTGTTGTTG |
R90A | F | CAACAACTTACAGGCTATTCGTGAACTGAC |
| Â | R | GTCAGTTCACGAATAGCCTGTAAGTTGTTG |
R90A | F | CAACAACTTACAGACTATTCGTGAACTGAC |
| Â | R | GTCAGTTCACGAATAGTCTGTAAGTTGTTG |