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Table 3 Distribution of the B. hyodysenteriae plasmid genes amongst the virulent and “avirulent” strains determined by GeneChip microarray and PCR

From: Absence of a set of plasmid-encoded genes is predictive of reduced pathogenic potential in Brachyspira hyodysenteriae

Gene (position on plasmid)

Putative function

Virulent strains

Avirulent strains

B204

BW1

NSW5

NSW15

Q17

Vic 2

A1

B78

B234

B6933

FM88.90

R301

SA2206

VS1

BHWA1_02666 (1)

glycosyltransferase

P

P

P

P

P

P

A

P

P

P

A

P

P

P

BHWA1_02667 (2)

NAD dependent epimerase/dehydratase

P

P

P

P

P

P

A

P

P

P

A

P

P

P

BHWA1_02668 (3)

glycosyl transferase, family 2

P

P

P

P

P

P

A

P

P

P

A

P

P

P

BHWA1_02669 (4)

glycosyl transferase, group 1-like protein

P

P

P

P

P

P

A

P

P

P

A

P

P

P

BHWA1_02670 (5)

glycosyl transferase, group 1

P

P

P

P

P

P

A

P

P

P

A

P

P

P

BHWA1_02671 (6)

glycosyl transferase

P

P

P

P

P

P

A

P

P

P

A

P

P

P

BHWA1_02672 (7)

hydrolase (HAD superfamily) protein

P

P

P

P

P

P

P

P

P

P

A

P

P

P

BHWA1_02673 (8)

hydrolase (HAD superfamily) protein

P

P

P

P

P

P

P

P

P

P

A

P

P

P

BHWA1_02674 (9)

Radical SAM domain protein

P

P

P

P

P

P

P

P

P

P

A

P

P

P

BHWA1_02675 (10)

Radical SAM domain protein

P

P

P

P

P

P

P

P

P

P

A

P

P

P

BHWA1_02676 (11)

Radical SAM domain protein

P

P

P

A

P

P

A

A

A

A

A

A

A

A

BHWA1_02677 (12)

Radical SAM domain protein

P

P

P

A

P

P

A

A

A

A

A

A

A

A

BHWA1_02678 (13)

Radical SAM domain protein

P

P

P

P

P

P

A

A

A

A

A

A

A

A

BHWA1_02679 (14)

glycosyl transferase, group 1-like protein

P

P

P

P

P

P

A

A

A

A

A

A

A

A

BHWA1_02680 (15)

NAD dependent epimerase

P

P

P

P

P

P

A

A

A

A

A

A

A

A

BHWA1_02681 (16)

dTDP-4-dehydrorhamnose 3,5-epimerase ( rfb C)

P

P

P

P

P

P

A

A

A

A

A

A

A

A

BHWA1_02682 (17)

Radical SAM domain protein

P

P

P

P

P

P

A

P

P

P

A

P

P

P

BHWA1_02683 (18)

Glucose-1-phosphate cytidylyltransferase (rfb F)

P

P

P

P

P

P

A

P

P

P

A

P

P

P

BHWA1_02684 (19)

plasmid partitioning protein (cds M)

P

P

P

P

P

P

A

P

P

P

A

P

P

P

BHWA1_02685 (20)

hypothetical protein

P

P

P

P

P

P

A

P

P

P

A

P

P

P

BHWA1_02686 (21)

replicative DNA helicase

P

P

P

P

P

P

A

P

P

P

A

P

P

P

BHWA1_02687 (22)

DNA primase-like protein

P

P

P

P

P

P

A

P

P

P

A

P

P

P

BHWA1_02688 (23)

integrase

P

P

P

P

P

P

A

P

P

P

A

P

P

P

BHWA1_02689 (24)

alpha-1,2-fucosyltransferase

P

A

A

P

A

P

A

P

P

P

A

A

P

P

BHWA1_02690 (25)

lipopolysaccharide biosynthesis protein-like protein

P

P

P

P

P

P

A

P

P

P

A

P

P

P

BHWA1_02691 (26)

dTDP-D-glucose 4,6-dehydratase (rfb B)

P

P

P

P

P

P

A

P

P

P

A

P

P

P

BHWA1_02692 (27)

glucose-1-phosphate thymidylyltransferase (rfb A)

P

P

P

P

P

P

A

P

P

P

A

P

P

P

BHWA1_02693 (28)

dTDP-4-keto-L-rhamnose reductase (rfb D)

P

P

P

P

P

P

A

P

P

P

A

P

P

P

BHWA1_02694 (29)

dTDP-4-dehydrorhamnose 3,5-epimerase (rfb C)

P

P

P

P

P

P

A

P

P

P

A

P

P

P

BHWA1_02695 (30)

hypothetical protein

P

P

P

P

P

P

A

P

P

P

A

P

P

P

BHWA1_02696 (31)

glycosyltransferase

P

P

P

P

P

P

A

P

P

P

A

P

P

P

  1. The names of seven of the plasmid genes that were not on the microarray (genes 1–3; 7–10) but were amplified by PCR are marked in bold. The microarray results for the four genes (genes 13–16) that were absent in the “avirulent” strains but present in the virulent strains are italicised. Genes 11 and 12 also were absent in the “avirulent” strains and were present in five of the six virulent strains (NSW15 being the exception).
  2. P represents gene present, A represents gene absent. Strains A1 and FM88-90 lacked all 31 plasmid genes.
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Veterinary Research

ISSN: 1297-9716