Table 2 PCR primers for amplification of the four genes on the plasmid that were absent in the “avirulent” B. hyodysenteriae strains but present in all the virulent strains
Primer nameb | Sequence (5′ – 3′) | Product size |
|---|---|---|
BHWA1_02678a-F | tagaaacggtaatcccattag | 322 bp |
BHWA1_02678a-R | taaaagcgaagcattagtagtag | |
BHWA1_02678b-F | tgataaagtaaatagggtagatactactac | 420 bp |
BHWA1_02678b-R | aaatacataaggacaaaccattac | |
BHWA1_02678c-F | ttttgctgaaatggtagagtatg | 558 bp |
BHWA1_02678c-R | tatagtttttactgattctttattgacatc | |
BHWA1_02679a-F | ttggtggaggagttggtac | 237 bp |
BHWA1_02679a-R | tgataaagaagaggatgattcc | |
BHWA1_02679b-F | tcaggtataaatccgcctaatg | 495 bp |
BHWA1_02679b-R | caggtactaaaccagcagtcatag | |
BHWA1_02679c-F | ggttttatatgtaagaatgaagatg | 329 bp |
BHWA1_02679c-R | cttagaatttgaagtccagtttg | |
BHWA1_02680a-F | gaagtttttggcataggaac | 289 bp |
BHWA1_02680a-R | tcattttcttttaatggtgtatc | |
BHWA1_02680b-F | aaaaggctatttagaatctgaag | 280 bp |
BHWA1_02680b-R | cattatctccataagtatagaaaactctac | |
BHWA1_02680c-F | tggtgatatagcaaaaagtatagc | 183 bp |
BHWA1_02680c-R | ccctacaataattttaggttcttcag | |
BHWA1_02681a-F | gtgggaaaaataaatctgaag | 345 bp |
BHWA1_02681a-R | gcttaattctacagtaaaatgctg | |
BHWA1_02681b-F | gtaatgatgaacttaaaaaatcaggtattg | 266 bp |
BHWA1_02681b-R | attccatgagccacataaggag | |
BHWA1_02681c-F | aaacagtcaaatatgagttatagtgc | 391 bp |
BHWA1_02681c-R | aaatctggtatacttttatccttttc |