Serum of Pregsure© BVD vaccinated dams contain alloantibodies. Total IgG alloantibody binding of MDBK cells was measured in serum of i) dams not vaccinated with Pregsure© BVD (BNP-VAcc-) ii) Pregsure© BVD vaccinated non-BNP dams (BNP-Vacc+) and iii) Pregsure© BVD vaccinated BNP dams (BNP + Vacc+) using flow cytometry. The black bars denote the mean Geometric Mean Fluorescent Intensity (GMFI). Results were compared by two tailed simple T-tests for unequal variance. To adjust for multiple comparisons, the False Discovery Rate (FDR) was controlled at 5% using the principle from Benjamini and Hochberg . The largest P-value lower than its FDR-derived significance threshold and all P-values smaller are significant and are depicted by an asterisk (*).